Original research papers

1. Deciphering Solution and Gas-Phase Interactions between Peptides and Lipids by Native Mass Spectrometry
   T Kundlacz, C Schmidt (2023)
   Analytical Chemistry. 95(47):17292-17299.
2. The Prenucleation Equilibrium of the Parathyroid Hormone Determines the Critical Aggregation Concentration and Amyloid Fibril Nucleation
   B Voigt, T Bhatia, J Hesselbarth, M Baumann, C Schmidt, M Ott, J Balbach (2023)
   ChemPhysChem 24(19):e202300439.
   
3. Mass Spectrometry Uncovers Intermediates and Off-pathway Complexes for SNARE complex assembly
   J Hesselbarth, C Schmidt (2023)
   Communications Biology 6(1):198.
4. Cryo-Electron Microscopy Snapshots of Eukaryotic Membrane Proteins in Native Lipid-Bilayer Nanodiscs
   K Janson, F Kyrilis, C Tüting, M Alfes, M Das, TK Träger, C Schmidt, F Hamdi, C Vargas, S Keller, A Meister, PL Kastritis (2022)
   Biomacromolecules 23(12):5084-5094.
5. Quantitative proteomics and in-cell cross-linking reveal cellular reorganisation during early neuronal differentiation of SH-SY5Y cells
   M Barth, A Toto Nienguesso, A Navarrete Santos, C Schmidt (2022)
   Communications Biology 5(1):551.
6. Viral immune evasins impact antigen presentation by allele-specific trapping of MHC I at the peptide-loading complex
   S Sethumadhavan, M Barth, RM Spaapen, C Schmidt, S Trowitzsch, R Tampé (2022)
   Scientific Reports 12(1):1516.
7. Thin-layer Chromatography and Coomassie Staining of Phospholipids for Fast and Simple Lipidomics Sample Preparation
   T Hofmann, M Barth, A Meister, PL Kastritis, C Schmidt (2021)
   Analysis & Sensing 1(4):171-179. (Cover article)
8. Assembly defects of the human tRNA splicing endonuclease contribute to impaired pre-tRNA processing in pontocerebellar hypoplasia
   S Sekulovski*, P Devant*, S Panizza*, T Gogakos, A Pitiriciu, K Heitmeier, EP Ramsay, M Barth, C Schmidt, S Weitzer, T Tuschl, F Baas, J Martinez§, S Trowitzsch§ (2021)
   Nature communications 12(1):5610.
9. A Nanoscale Model System for the Human Myelin Sheath
   M Hoffmann, D Haselberger, T Hofmann, L Müller, K Janson, A Meister, S Keller, PL Kastritis, C Schmidt, D Hinderberger (2021)
   Biomacromolecules 22(9):3901-3912.
10. Effects of non-ionic and zwitterionic detergents on soluble proteins during native mass spectrometry experiments
    T Kundlacz*, J Bender*, C Schmidt (2021)
    International Journal of Mass Spectrometry 468:116652.
11. Liposomes as carriers of membrane-associated proteins and peptides for mass
    spectrometric analysis
    M Frick, C Schwieger, C Schmidt (2021)
    Angewandte Chemie International Edition 60(20):11523-11530. (Cover article)
12. Cross-linking mass spectrometry uncovers protein interactions and functional
    assemblies in synaptic vesicle membranes
    S Wittig, M Ganzella, M Barth, S Kostmann, D Riedel, A Perez-Lara, R Jahn, C Schmidt (2021)
    Nature communications. 12(1):858.
13. Alternatively spliced isoforms of AUF1 differentially regulate miRNA-mRNA interaction through its YGG motif
    L Sänger, J Bender, K Rostowski, R Golbik, H Lilie, C Schmidt, S-E Behrens, S Friedrich (2020)
    RNA Biology 18(6):843-853.
14. Polydisperse molecular architecture of connexin 26/30 heteromeric hemichannels revealed by AFM imaging
    PA Naulin, B Lozano, C Fuentes, Y Liu, C Schmidt, AL Harris, JE Contreras, NP Barrera (2020)
    The Journal of Biological Chemistry. 295(49):16499-16509.
15. Evaluation of NHS-Acetate and DEPC labelling for determination of solvent accessible
    amino acid residues in protein complexes
    M Barth, J Bender, T Kundlacz, C Schmidt (2020)
    Journal of Proteomics 222:103793.
16. Stoichiometries and stability of CRISPR-Cascade complexes with varying spacer length revealed by native mass spectrometry
    S Wittig, I Songailiene, C Schmidt (2020)
    Journal of The American Society for Mass Spectrometry. 31(3):538-546.
17. The CroCo cross-link converter: a user-centred tool to convert results from crosslinking mass spectrometry experiments
    J Bender, C Schmidt (2020)
    Bioinformatics 36(4):1296-1297.
18. Protein-lipid interactions stabilise the oligomeric state of Bor1p from Saccharomyces cerevisiae
    E Pyle, C Guo, T Hofmann, C Schmidt, O Ribeiro, A Politis§, B Byrne§ (2019)
    Analytical Chemistry 91(20):13071-13079.
19. Control of p21Cip by BRAP is Critical for Cardiomyocyte Cell Cycle Progression and Survival
    C Volland, P Schott, M Didié, J Männer, B Unsöld, K Toischer, C Schmidt, H Urlaub, K Nickels, R Knöll, A Schmidt, K Guan, G Hasenfuß, T Seidler (2020)
    Cardiovascular Research 116(3):592-604.
20. Decision making in Cascade complexes harboring crRNAs of altered length
    I Songailiene, M Rutkauskas, T Sinkunas, E Manakova, S Wittig, C Schmidt, V Siksnys§, R Seidel§ (2019)
    Cell Reports 28(12):3157-3166.
21. Structural basis of Cullin-2 RING E3 ligase regulation by the COP9 signalosome
    SV Faull, AMC Lau, C Martens, Z Ahdash, H Yebenes, C Schmidt, F Beuron, NB Cronin, EP Morris§, A Politis§ (2019)
    Nature communications 10:3814.
22. Instrument response of phosphatidylglycerol lipids with varying fatty acyl chain length in nano-ESI shotgun experiments
    T Hofmann, C Schmidt (2019)
    Chemistry and Physics of Lipids 223:104782.
23. Cross-linking/Mass Spectrometry: A Community-Wide, Comparative Study Towards Establishing Best Practice Guidelines
    C Iacobucci*, C Piotrowski*, R Aebersold, BC Amaral, P Andrews, C Borchers, NI Brodie, JE Bruce, S Chaignepain, JD Chavez, S Claverol, J Cox, G Degliesposti, M-Q Dong, N Edinger, C Emanuelsson, M Gay, M Götze, FC Gozzo, C Gutierrez, C Haupt, AJR Heck, F Herzog, L Huang, MR Hoopmann, N Kalisman, O Klykov, Z Kukačka, F Liu, MJ MacCoss, K Mechtler, R Mesika, RL Moritz, N Nagaraj, V Nesati, R Ninnis, P Novák, FJ O’Reilly, M Pelzing, E Petrotchenko, L Piersimoni, M Plasencia, T Pukala, KD Rand, J Rappsilber, D Reichmann, C Sailer, CP Sarnowski, RA Scheltema, C Schmidt, D Schriemer, DC Schriemer, Y Shi, JM Skehel, M Slavin, F Sobott, V Solis-Mezarino, H Stephanowitz, F Stengel, CE Stieger, M Trnka, M Vilaseca, R Viner, Y Xiang, S Yilmaz, A Zelter, D Ziemanowicz, A Leitner§, A Sinz§ (2019)
    Analytical Chemistry 91(11):6953-6961.
24. Structural and functional analysis of the human pyruvate dehydrogenase complex suggest a “division-of-labor” mechanism by local E1 and E3 clusters
    S Prajapati*, D Haselbach*, S Wittig, MS Patel, A Chari, C Schmidt*§, H Stark§, K Tittmann§ (2019)
    Structure 27(7):1124-1136.
25. Oligomerisation of Synaptobrevin-2 Studied by Native Mass Spectrometry and Chemical Cross-Linking
    S Wittig, C Haupt, W Hoffmann, S Kostmann, K Pagel, C Schmidt (2019)
    Journal of The American Society for Mass Spectrometry 30(1):149-160.
26. A novel sample preparation strategy for shotgun lipidomics of phospholipids employing multilamellar vesicles
    M Frick, T Hofmann, C Haupt, C Schmidt (2018)
    Analytical and Bioanalytical Chemistry 410(18):4253-4258.
27. Combining chemical cross-linking and mass spectrometry of intact protein complexes to study the architecture of multi-subunit protein assemblies
    C Haupt*, T Hofmann*, S Wittig*, S Kostmann, A Politis, C Schmidt (2017)
    Journal of Visualised Experiments 129:56747.
28. Structure of the human MHC-I peptide-loading complex
    A Blees, D Januliene, T Hofmann, N Koller, C Schmidt, S Trowitzsch§, A Möller§, R Tampé§ (2017)
    Nature 551(7681):525-528.
29. Structure based biophysical characterization of the PROPPIN Atg18 shows Atg18 oligomerization upon membrane binding
    A Scacioc, C Schmidt§, T Hofmann, H Urlaub, K Kühnel§, Á Pérez-Lara§ (2017)
    Scientific Reports 7:14008.
30. Accommodating protein dynamics in the modelling of chemical cross-links
    M Degiacomi, C Schmidt, AJ Baldwin, JLP Benesch§ (2017)
    Structure 25(11):1751-1757.
31. Small molecule-induced soluble oligomers of α-synuclein with helical structure
    L Fonseca-Ornelas, C Schmidt, AR Camacho-Zarco, CO Fernandez, S Becker, M Zweckstetter (2017)
    Chemistry 23(53):13010-13014.
32. Acetylation and phosphorylation control both local and global stability of the chloroplast F1 ATP synthase
    C Schmidt§, V Beilsten-Edmands, S Mohammed, CV Robinson§ (2017)
    Scientific Reports 7:44068.
33. Surface Accessibility and Dynamics of Macromolecular Assemblies Probed by Covalent Labeling Mass Spectrometry and Integrative Modeling
    C Schmidt, JA Macpherson, AM Lau, KW Tan, F Fraternali, A Politis (2017)
    Analytical Chemistry 89(3):1459-1468.
34. Atomic model for the membrane-embedded VO motor of a eukaryotic V-ATPase
    MT Mazhab-Jafari, A Rohou, C Schmidt, SA Bueler, S Benlekbir, CV Robinson, JL Rubinstein (2017)
    Nature 539(7627):118-122.
35. Dimer interface of bovine cytochrome c oxidase is influenced by local posttranslational modifications and lipid binding
    I Liko, MT Degiacomi, S Mohammed, S Yoshikawa, C Schmidt§, CV Robinson§ (2016)
    PNAS 113(29):8230-8235.
36. Uncovering the Early Assembly Mechanism for Amyloidogenic β2-Microglobulin Using Cross-linking and Native Mass Spectrometry
    Z Hall, C Schmidt, A Politis (2016)
    The Journal of Biological Chemistry 291(9):4626-4637.
37. eIF2 interactions with initiator tRNA and eIF2B are regulated by post-translational modifications and conformational dynamics
    V Beilsten-Edmands, Y Gordiyenko, JCK Kung, S Mohammed, C Schmidt§, CV Robinson§ (2015)
    Cell Discovery 1:15020.
38. Hsp70 Forms Antiparallel Dimers Stabilized by Post-translational Modifications to Position Clients for Transfer to Hsp90
    N Morgner*, C Schmidt*, V Beilsten-Edmands*, IO Ebong, NA Patel, E Kirschke, S Daturpalli, SE Jackson, D Agard, CV Robinson (2015)
    Cell Reports 11(5):759-769.
39. Topological models of heteromeric protein assemblies from mass spectrometry: Application to the yeast eIF3:eIF5 complex
    A Politis*, C Schmidt*, E Tjioe, AM Sandercock, K Lasker, Y Gordiyenko, D Russel, A Sali§, CV Robinson§ (2015)
    Chemistry & Biology 22(1):117-128.
40. A comparative cross-linking strategy to probe conformational changes in protein
    complexes
    C Schmidt, CV Robinson (2014)
    Nature protocols 9(9):2224-2236.
41. eIF2B is a decameric guanine nucleotide exchange factor with a γ2ε2 tetrameric core
    Y Gordiyenko*, C Schmidt*, MD Jennings, D Matak-Vinkovic, GD Pavitt§, CV Robinson§ (2014)
    Nature Communications 5:3902.
42. Relative quantification of pre-catalytic and catalytically active spliceosomes: a comparative study of metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count
    C Schmidt*, M Gronborg*, J Deckert, S Bessonov, T Conrad, R Lührmann, H Urlaub (2014)
    RNA 20(3):406-420.
43. Crystal Structure of the Human Short Coiled Coil Protein and Insights into SCOC-FEZ1 Complex Formation
    C Behrens, B Binotti, C Schmidt, CV Robinson, JJ Chua, K Kühnel (2013)
    PLoS One 8(10):e76355.
44. Comparative cross-linking and mass spectrometry of an intact F-type ATPase suggest
    a role for phosphorylation
    C Schmidt, M Zhou, H Marriott, N Morgner, A Politis, CV Robinson (2013)
    Nature Communications 4:1985.
45. An automated in-gel digestion/iTRAQ-labeling workflow for robust quantification of gel- separated proteins
    C Schmidt, D Hesse, M Raabe, H Urlaub, O Jahn (2013)
    Proteomics 13(9):1417-1422.
46. Determination of protein stoichiometry within protein complexes using absolute quantification and multiple reaction monitoring
    C Schmidt, C Lenz, M Grote, R Lührmann, H Urlaub (2010)
    Analytical Chemistry 82:2784-2796.